Under The Light Microscopic View of Compact Bone
Identifying histological features on a compact bone histology slide involves examining the tissue under a microscope. Here are key points to look for when identifying structures in compact bone histology slides:
Osteons (Haversian Systems):
- The fundamental structural units of compact bone.
- Consist of concentric layers of lamellae surrounding a central canal (Haversian canal).
Haversian Canal:
- The central canal within each osteon that contains blood vessels, nerves, and lymphatics.
- Provides a pathway for nutrient exchange and waste removal.
Concentric Lamellae:
- Rings or layers of bone matrix surrounding the Haversian canal within an osteon.
- Composed mainly of collagen fibers and mineralized matrix (hydroxyapatite crystals).
Lacunae:
- Small spaces between the lamellae that house mature bone cells called osteocytes.
Canaliculi:
- Microscopic channels that connect lacunae and allow communication between osteocytes.
- Facilitate the exchange of nutrients and waste products.
Perforating (Volkmann's) Canals:
- Channels that run perpendicular to the central canals, connecting multiple osteons.
- Provide additional pathways for blood vessels and nerves to reach deeper layers of compact bone.
Interstitial Lamellae:
- Fragments of lamellae that are remnants of previous osteons and fill the spaces between intact osteons.
Circumferential Lamellae:
- Layers of bone matrix that encircle the outer and inner surfaces of compact bone.
- Provide additional structural support.
Endosteum:
- A thin layer of connective tissue lining the medullary cavity and the inner surfaces of compact bone.
- Contains osteoprogenitor cells involved in bone remodeling and repair.
Periosteum:
- The outer covering of bones, not always visible in compact bone sections.
- Composed of connective tissue and involved in bone growth and repair.
Blood Vessels:
- Identify blood vessels within the Haversian canals, perforating canals, and periosteum, supplying nutrients and oxygen to bone cells.
- above image taken under the light microscope
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